Differential activation of cAMP- and cGMP-dependent protein kinases by cyclic purine and pyrimidine nucleotides

作者：Wolter Sabine; Golombek Marina; Seifert Roland^*

来源：Biochemical and Biophysical Research Communications, 2011, 415(4): 563-566.

DOI：10.1016/j.bbrc.2011.10.093

• cCMP
• cUMP
• PKA
• PKG
• Second messenger

The cyclic purine nucleotides cAMP and cGMP are well-characterized second messengers and activators of PKA and PKG, respectively. In contrast, the functions of the cyclic pyrimidine nucleotides cCMP and cUMP are poorly understood. cCMP induces relaxation of smooth muscle via PKGI, and phosphodiesterases differentially hydrolyze cNMPs. Here, we report that cNMPs differentially activate PKA isoforms and PKGI alpha. The combination of cCMP with cAMP reduced the EC(50) of cAMP for PKA. PKGI alpha exhibited higher specificity for the cognate cNMP than PKA. Our data support a role of cCMP and cUMP as second messengers.

• 出版日期2011-12-2