N-acetylcysteine leads to greater ovarian protection than enoxaparin sodium in a rat ovarian torsion model

作者：Ersoy Gulcin Sahin^*; Eken Meryem; Tal Reshef; Oztekin Deniz; Devranoglu Belgin; Kaygusuz Ecmel Isik; Cevik Ozge

来源：Reproductive BioMedicine Online, 2016, 33(1): 93-101.

DOI：10.1016/j.rbmo.2016.03.009

• anti-Mullerian hormone
• enoxaparin sodium
• N-acetylcysteine
• ovarian ischaemia-reperfusion
• ovarian reserve
• torsion

This study evaluated the effects of N-acetylcysteine (NAC) and enoxaparin on ovarian tissue preservation, ovarian reserve and oxidative damage following ovarian torsion/detorsion injury. Rats were divided into four groups (n = 6/group): control; ischaemia/reperfusion (I/R); I/R + NAC; I/R + enoxaparin. Twenty-four hours after detorsion, ovarian tissues were collected for histopathological analysis and measurement of tissue 8-OHdG, GSH, MDA, MPO and SOD concentrations, as well as pre- and post-operative circulating AMH concentrations. Administration of NAC resulted in more pre-antral follicles compared with enoxaparin treatment and haemorrhage and follicle cell degeneration were more pronounced in I/R + enoxaparin group than I/R + NAC group. Both NAC and enoxaparin led to a significant reduction in ovarian tissue 8-OHdG (P = 0.004 and P = 0.01, respectively) and MPO (P = 0.013 and P = 0.023, respectively) concentrations compared with I/R group, indicating a protective effect against I/R oxidative damage. Only NAC-treated animals showed a significant increase in GSH and SOD concentrations and decrease in MDA concentrations compared with I/R group (P = 0.007, P = 0.024 and P = 0.026, respectively). These results indicate that NAC is more effective than enoxaparin in minimizing ovarian damage and preserving ovarian reserve following ovarian torsion.