Scattering detection using a photonic-microfluidic integrated device with on-chip collection capabilities

作者：Watts Benjamin R; Zhang Zhiyi^*; Xu Chang Qing; Cao Xudong; Lin Min

来源：Electrophoresis, 2014, 35(2-3): 271-281.

DOI：10.1002/elps.201300195

• Integrated optics
• Lab-on-a-chip
• Microfluidic devices
• Optofluidics
• Scatter detection

SU-8-based photonic-microfluidic integrated devices with on-chip beam shaping and collection capabilities were demonstrated in a scattering detection and counting application. Through the proper deployment of the tailored beam geometries via the on-chip excitation optics, excellent CV values were measured for 1, 2, and 5 m blank beads, 16.4, 11.0, and 12.5%, respectively, coupled with a simple free-space optical detection scheme. The performance of these devices was found dependent on the combination of on-chip, lens-shaped beam geometry and bead size. While very low CVs were obtained when the combination was ideal, a nonideal combination could still result in acceptable CVs for flow cytometry; the reliability was confirmed via devices being able to resolve separate populations of 2.0 and 5.0 m beads from their mixture with low CV values of 15.9 and 18.5%, respectively. On-chip collection using integrated on-chip optical waveguides was shown to be very reliable in comparison with a free-space collection scheme, yielding a coincident rate of 94.2%. A CV as low as 19.2% was obtained from the on-chip excitation and collection of 5 m beads when the on-chip lens-shaped beam had a 6.0-m beam waist.

• 出版日期2014-2