Antioxidant synergism between ethanolic Centella asiatica extracts and alpha-tocopherol in model systems

作者：Thoo Yin Yin; Abas Faridah; Lai Oi Ming; Ho Chun Wai; Yin Jie; Hedegaard Rikke V; Skibsted Leif H; Tan Chin Ping^*

来源：Food Chemistry, 2013, 138(2-3): 1215-1219.

DOI：10.1016/j.foodchem.2012.11.013

• Synergism
• Ultrasound
• alpha-Tocopherol
• Isobolographic analysis

The synergistic antioxidant effects of ethanolic extracts of Centella asiatica (CE), and alpha-tocopherol have been studied. The types of interactions exhibited by CE and alpha-tocopherol combined at different ratios were measured using three assays: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical-scavenging capacity, the beta-carotene bleaching system and liposome peroxidation assays. Fixed-fraction isobolographic analysis was used to detect any inducement of the antioxidant activity compared with the individual activities of CE and alpha-tocopherol. Of all synergistic combinations of CE and alpha-tocopherol, only fraction 2/3 showed the synergistic combination that fits well in three different assays and can be explained by the regeneration of alpha-tocopherol by CE despite the interaction effect of beta-carotene present in the analytical assay. This phenomenon involved complex interactions between CE and alpha-tocopherol to exhibit different degrees of interactions that eventually increased antioxidant activity.

• 出版日期2013-5-15