Analysis of nerve agent metabolites from nail clippings by liquid chromatography tandem mass spectrometry

作者：Appel Amanda S; Logue Brian A^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2016, 1031: 116-122.

DOI：10.1016/j.jchromb.2016.07.034

• Soman
• Pinacolyl methylphosphonic acid
• Sarin
• Isopropyl methylphosphonic acid
• Liquid-chromatography mass-spectrometry
• Long-term chemical warfare agent biomarker
• Nerve agent exposure

While several methods for the bioanalysis of nerve agents or their metabolites have been developed for the verification of nerve agent exposure, these methods are generally limited in the amount of time after an exposure that markers of exposure can be detected (due to rapid metabolism from biological matrices). In this study, a method for the analysis of nerve agent hydrolysis products from nail clippings was developed to allow evaluation of nails as a long-term repository of these markers. Pinacolyl methylphosphonic acid (PMPA) and isopropyl methylphosphonic acid (IMPA) were extracted from nail samples with N,N-dimethylformamide and subsequently analyzed by liquid chromatography-tandem mass spectrometry. Limits of detection for PMPA and IMPA were 03 mu g/kg and 7.5 mu g/kg and linear ranges were 0.75-300 mu g/kg and 30-1500 mu g/kg, respectively. Precision was within 10% and 8% for PMPA and IMPA, respectively, and accuracy was 100 +/- 12% for both analytes. The approach presented here is complementary to current methods for nerve agent exposure verification, and should allow for long-term determination of nerve agent poisoning.

• 出版日期2016-9-15