Differential expression of proteins in the midgut of Anopheles albimanus infected with Plasmodium berghei

作者：Serrano Pinto Vania^*; Acosta Perez Maribel; Luviano Bazan Darwin; Hurtado Sil Gerardo; Batista Cesar V F; Martinez Barnetche Jesus; Lanz Mendoza Humberto

来源：Insect Biochemistry and Molecular Biology, 2010, 40(10): 752-758.

DOI：10.1016/j.ibmb.2010.07.011

• Anopheles albimanus
• Malaria
• Midgut protein
• Mosquitoes
• Plasmodium berghei
• Proteomics
• Transmission
• Two-dimensional electrophoresis

The main vector for transmission of malaria in Mexico is the Anopheles albimanus mosquito. The midgut of disease-transmitting mosquitoes carries out a variety of functions that are related to blood feeding. We analyzed the midgut of A. albimanus infected with Plasmodium berghei (resistant mosquito) using a proteomic approach to identify putative short peptides that are enriched in the midgut after blood feeding. Mosquito midguts were analyzed by two-dimensional electrophoresis to determine the changes in protein profiles. We identified 21 spot proteins that are differentially expressed in the blood of mosquitoes during the immune challenge. Molecular weight of the spots varied from 13 to 36 kDa, with a broad isoelectric point range of 3.92-8.90. We identified the differentially expressed proteins using mass spectrometry and constructed a proteomic data base of the A. albimanus midgut with diverse functions, some of them proteins with digestive and immunologic functions. Identification of these proteins may have important implications for understanding the blood meal digestion process, as well as developing novel vector control strategies and understanding parasite vector interactions.

• 出版日期2010-10