科研之友
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摘要
Objectiv e: To explore antagonistic key molecule of mitochondrial carnitine shuttle system on effects of hepatic fatty acid metabolism. Methods: Wild type C57BJ/6j(wt/wt) mice were intraperitoneally injected 0.05% of carnitine analogue [(3-(2,2,2-trimethyl hydrazinium, W/W/d) propionate dehydrate,THP)] to antagonistic carnitine, and the control mice with equal quantity saline (0.85%, W/V), and sacrificed with ether anesthesia after two weeks, and the livers were strained with oil red for observing hepatic fatty accumulation and the levels of different type carnitines including total, free, long, and short-chain were quantitatively analyzed in liver tissues by radiometric method. Results: After the mice injected with THP, the liver weight in the injected group was significantly increased(P <0.05) than that in the control group, with the fatty accu-mulation in hepatocytes. The hepatic specific concentrations(nmol/g wet liver) in the injected group were significantly higher (P <0.05) than those in the control, with (392.16±53.08) vs (323.78±51.29) in total, (252.28±28.21) vs (151.46±37.81) in free, and (78.32±9.51) vs (65.80±7.73) in long chain carnitine, and remarkedly decreased in short-chain carnitine (61.56±23.28 vs 106.92±41.16), respectively. Conclusion: Carnitine is located on inner membrance of mitochondria and one of key enzymes limited β-oxidation process.
- 出版日期2014
- 单位南通大学
