Acetyl-CoA production by encapsulated pyruvate ferredoxin oxidoreductase in alginate hydrogels

作者：Takenaka Makoto; Yoon Ki Seok; Matsumoto Takahiro; Ogo Seiji^*

来源：Bioresource Technology, 2017, 227: 279-285.

DOI：10.1016/j.biortech.2016.12.051

• Acetyl-CoA production
• Pyruvate ferredoxin oxidoreductase
• Encapsulation
• Immobilization
• Kinetic parameters
• Purification and characterization

Pyruvate ferredoxin oxidoreductase from Citrobacter sp. S-77 (PFORS77) was purified in order to develop a method for acetyl-CoA production. Although the purified PFORS77 showed high O-2-sensitivity, the activity could be remarkably stabilized in anaerobic conditions. PFORS77 was effectively immobilized on ceramic hydroxyapatite (PFORS77-HA) with an efficiency of more than 96%, however, after encapsulation of PFORS77-HA in alginate, the rate of catalytic acetyl-CoA production was highly reduced to 36% when compared to that of the free enzyme. However, the operational stability of the PFORS77-HA in alginate hydrogels was remarkable, retaining over 68% initial activity even after ten repeated cycles. The results suggested that the PFORS77-HA hydrogels have a high potential for biotechnological application.

• 出版日期2017-3