Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus

作者：Silva Evangelista Claudia Carolina; Guidelli Giovanna Vieira; Borges Gustavo; Araujo Thais Fenz; Jorge de Souza Tiago Alves; da Costa Neves Ubiraci Pereira; Tunnacliffe Alan; Pereira Tiago Campos^*

来源：Genetics and Molecular Biology, 2017, 40(4): 790-802.

DOI：10.1590/1678-4685-GMB-2017-0030

• peroxiredoxin
• kinase
• RNAi
• proteostasis

The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holoanhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways. This suggests that multiple genes contribute to anhydrobiosis in P. superbus.

• 出版日期2017-12