A Novel CO-Responsive Transcriptional Regulator and Enhanced H-2 Production by an Engineered Thermococcus onnurineus NA1 Strain

作者：Kim Min Sik; Choi Ae Ran; Lee Seong Hyuk; Jung Hae Chang; Bae Seung Seob; Yang Tae Jun; Jeon Jeong Ho; Lim Jae Kyu; Youn Hwan; Kim Tae Wan; Lee Hyun Sook^*; Kang Sung Gyun

来源：Applied and Environmental Microbiology, 2015, 81(5): 1708-1714.

DOI：10.1128/AEM.03019-14

Genome analysis revealed the existence of a putative transcriptional regulatory system governing CO metabolism in Thermococcus onnurineus NA1, a carboxydotrophic hydrogenogenic archaeon. The regulatory system is composed of CorQ with a 4-vinyl reductase domain and CorR with a DNA-binding domain of the LysR-type transcriptional regulator family in close proximity to the CO dehydrogenase (CODH) gene cluster. Homologous genes of the CorQR pair were also found in the genomes of Thermococcus species and "Candidatus Korarchaeum cryptofilum" OPF8. In-frame deletion of either corQ or corR caused a severe impairment in CO-dependent growth and H-2 production. When corQ and corR deletion mutants were complemented by introducing the corQR genes under the control of a strong promoter, the mRNA and protein levels of the CODH gene were significantly increased in a Delta CorR strain complemented with integrated corQR (Delta CorR/corQR up arrow) compared with those in the wild-type strain. In addition, the Delta CorR/corQR up arrow strain exhibited a much higher H-2 production rate (5.8-fold) than the wild-type strain in a bioreactor culture. The H-2 production rate (191.9 mmol liter(-1) h(-1)) and the specific H-2 production rate (249.6 mmol g(-1) h(-1)) of this strain were extremely high compared with those of CO-dependent H-2-producing prokaryotes reported so far. These results suggest that the corQR genes encode a positive regulatory protein pair for the expression of a CODH gene cluster. The study also illustrates that manipulation of the transcriptional regulatory system can improve biological H-2 production.

• 出版日期2015-3