Use of partial least-squares discriminant analysis to study the effects of gradual scale-up of culture, and optimization of bioreactor angle and aeration volume on culture of Panax quinquefolium L. adventitious roots in a 5-L balloon-type bubble bioreactor

作者：Wang, Qin; Wang, Juan; Li, Jing; Man, Shuli; Gao, Wenyuan^*

来源：Research on Chemical Intermediates, 2015, 41(9): 6707-6720.

DOI：10.1007/s11164-014-1770-y

• Panax quinquefolium
• PLS-DA
• Polysaccharide
• Ginsenoside
• Adventitious roots
• Bioreactor

Gradual scale-up culture of adventitious roots of Panax quinquefolium L. has been successfully achieved. Production of ginsenosides and polysaccharides was enhanced in a bioreactor compared with flask-type culture. The conditions for culture of adventitious roots were optimized. Partial least-squares discriminant analysis of the results showed growth rate and amounts of some compounds correlated positively with use of a 5-L balloon-type bubble bioreactor at an angle of 90 degrees and with an aeration volume of 0.5 v/v/min.

• 出版日期2015-9
• 单位天津科技大学; 天津大学