NMR detection of bifurcated hydrogen bonds in large proteins

作者：Liu Aizhuo^*; Lu Zhenwei; Wang Jifeng; Yao Lishan; Li Yue; Yan Honggao

来源：Journal of the American Chemical Society, 2008, 130(8): 2428-2429.

DOI：10.1021/ja710114r

Hydrogen bonds play critical roles in protein structure, stability, and function. Conventionally, hydrogen bonds are mainly determined by X-ray crystallography and NOE-based NMR spectroscopy in indirect manners. In recent years, it was demonstrated that hydrogen bonds can be directly detected through NMR measurements of trans-hydrogen-bond scalar coupling constants. Here we report across hydrogen-bond protium/deuterium isotope effects in a 35 kDA protein observed with the isotopomer-selective TROSY NMR technique (Liu at al. J. Biomol. NMR 2006, 36, 205-214; Liu at al. J. Magn. Reson. 2007, 186, 319-326) and show that such isotope effects can be used to detect a most common type of bifurcated hydrogen bonds, in which a heavy atom, usually oxygen, is involved in two hydrogen bonds, including a pair of bifurcated hydrogen bonds involving a bound water molecule.

• 出版日期2008-2-27