Aqueous-Level Turnover Frequency of Lipase in Organic Solvent

作者：Lee Eungyeong; Oh Yeonock; Choi Yoon Kyung; Kim Mahn Joo^*

来源：ACS Catalysis, 2014, 4(10): 3590-3592.

DOI：10.1021/cs500832e

• lipase
• activation
• surfactant
• dextrin-surfactant combination
• synergy effect

In this work, we explored the activation of a lipoprotein lipase from Burkholderia sp. by surfactants, sugars, and sugar surfactant combinations for enhancing its activity in organic solvent. The lipase was most strongly activated by the combination of dextrin and anionic surfactant 1. As a result, its turnover frequency in anhydrous toluene reached 420 s(-1), which was comparable to its aqueous counterpart. It was also found that a glucosamine-headed surfactant 8 as a molecular mimic of the dextrin-1 combination enhanced the turnover frequency of LPL to the aqueous level. As a rationale for such a high turnover frequency of lipoprotein lipase in anhydrous organic solvent, we speculate that the dextrin-1 couple or its mimic 8 could provide an efficient water-mimicking hydrogen bonding network around the enzyme in addition to the contribution as the oil surrogate, thus leading to a large increase of active enzyme molecules.

• 出版日期2014-10