A Nodule-Specific Protein Secretory Pathway Required for Nitrogen-Fixing Symbiosis

作者:Wang Dong; Griffitts Joel; Starker Colby; Fedorova Elena; Limpens Erik; Ivanov Sergey; Bisseling Ton; Long Sharon*
来源:Science, 2010, 327(5969): 1126-1129.
DOI:10.1126/science.1184096

摘要

The nitrogen-fixing symbiosis between Sinorhizobium meliloti and its leguminous host plant Medicago truncatula occurs in a specialized root organ called the nodule. Bacteria that are released into plant cells are surrounded by a unique plant membrane compartment termed a symbiosome. We found that in the symbiosis-defective dnf1 mutant of M. truncatula, bacteroid and symbiosome development are blocked. We identified the DNF1 gene as encoding a subunit of a signal peptidase complex that is highly expressed in nodules. By analyzing data from whole-genome expression analysis, we propose that correct symbiosome development in M. truncatula requires the orderly secretion of protein constituents through coordinated up-regulation of a nodule-specific pathway exemplified by DNF1.

  • 出版日期2010-2-26