Novel fluorescent probe for lead ion detection based on DNAzyme

作者：Wang Qing; Yang Xiao Hal; Wang Ling; Wang Ke Min^*; Zhao Xiang

来源：Chemical Journal of Chinese Universities, 2007, 28(12): 2270-2273.

• 17E DNAzyme
• lead
• fluorescence

A novel fluorescent probe for Pb2+ was obtained, in which the quencher modified 17E DNAzyme and the fluorophore-modified substrate strand were connected through 6 adenine deoxynucleotides. Due to the intramolecular hybridization between 17E DNAzyme and the substrate strand, the quencher was closed to the fluorophore and it resulted in fluorescence quenching. In the presence of Pb2+, the substrate strand was cleaved under the catalysis of enzyme strand, and the fluorophore-modified fragment was released, which resulted in the increase of fluorescence. Pb2+ could be rapidly detected at room temperature based on this principle, and the limits of detection were 10 nmol/L. For divalent metal ions, such as Zn2+, Mn2+, Co2+, Cd2+, Cu2+, Mg2+ and Ni2+, only Zn2+, Mn2+ and Cd2+ showed a slight increase of fluorescence intensity while all the other metal ions gave approximately background intensity. It is demonstrated that the novel fluo rescent probe has a good selectivity to Pb2+.

• 出版日期2007-12-10
• 单位湖南大学