Accelerated Solvent Extraction Coupled to GC-MS/MS for the Analysis of Atractylenolide in Atractylodes macrocephala

作者：Liao Qie Gen; Li Rui Li; Luo Lin Guang^*

来源：Chromatographia, 2012, 75(15-16): 931-935.

DOI：10.1007/s10337-012-2273-x

• Gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS)
• Accelerated solvent extraction (ASE)
• Atractylenolide (AO)
• Atractylodes macrocephala

The performance of accelerated solvent extraction (ASE) in the analysis of atractylenolide I (AO-I) and atractylenolide III (AO-III) in Atractylodes macrocephala rhizome samples was investigated and compared with that of Soxhlet extraction (SE) and ultrasonically assisted extraction in terms of yield, extraction time, reproducibility, and solvent consumption. The results indicated that although the highest yield was achieved by SE, ASE appears to be a promising alternative to classical methods because it is faster and requires less solvent, especially when investigating large batches of plant samples. The use of ethanol at a temperature of 100 A degrees C, performing two extraction cycles with a static duration of 10.0 min, represented the optimal ASE parameters. The extracts were analyzed by gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS).

• 出版日期2012-8
• 单位江西省农业科学院