A molecular method for the delivery of small molecules and proteins across the cell wall of algae using molecular transporters

作者：Hyman Joel M; Geihe Erika I; Trantow Brian M; Parvin Bahram^*; Wender Paul A

来源：Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(33): 13225-13230.

DOI：10.1073/pnas.1202509109

• cell penetrating peptides
• imaging
• protein delivery

Interest in algae has significantly accelerated with the increasing recognition of their potentially unique role in medical, materials, energy, bioremediation, and synthetic biological research. However, the introduction of tools to study, control, or expand the inner-workings of algae has lagged behind. Here we describe a general molecular method based on guanidinium-rich molecular transporters (GR-MoTrs) for bringing small and large cargos into algal cells. Significantly, this method is shown to work in wild-type algae that have an intact cell wall. Developed using Chlamydomonas reinhardtii, this method is also successful with less studied algae including Neochloris oleoabundans and Scenedesmus dimorphus thus providing a new and versatile tool for algal research.

• 出版日期2012-8-14