Application of pyrosequencing for Salmonella enterica rapid identification

作者：Li Xiujuan; Yang Fang; Gao Weili; Song Hongmei; Tian Huifang; Xu Baohong^*

来源：Journal of Microbiological Methods, 2012, 89(1): 49-52.

DOI：10.1016/j.mimet.2012.01.020

• Pyrosequencing
• Salmonella
• Rapid identification

Application of pyrosequencing of six Salmonella-specific genes as a rapid Salmonella identification method was tested. Primers for fins, hisJ and hilA had non-specific reactions with non-Salmonella strains. Primers for invA, iroB and fimY had specific PCR products and pyrosequences of Salmonella, suggesting that they were suitable for Salmonella rapid identification.

• 出版日期2012-4
• 单位河北师范大学