A NBD-based simple but effective fluorescent pH probe for imaging of lysosomes in living cells

作者：Cao, Xiang-Jian; Chen, Li-Na; Zhang, Xuan; Liu, Jin-Ting; Chen, Ming-Yu; Wu, Qiu-Rong; Miao, Jun-Ying^*; Zhao, Bao-Xiang^*

来源：Analytica Chimica Acta, 2016, 920: 86-93.

DOI：10.1016/j.aca.2016.03.029

• NBD
• Fluorescent probe
• Intracellular pH
• Cell imaging
• Lysosomes

NBDlyso with lysosome-locating morpholine moiety has been developed as a high selective and sensitive fluorescent pH probe. This probe can respond to acidic pH (2.0-7.0) in a short time (less than 1 min) and not almost change after continuously illuminated for an extended period by ultraviolet light. The fluorescence intensity of NBDlyso enhanced 100-fold in acidic solution, with very good linear relationship (R-2 = 0.996). The pK(a) of probe NBDlyso is 4.10. Therefore, NBDlyso was used to detect lysosomal pH changes successfully. Besides, X-ray crystallography was used to verify the structure of NBDlyso, and the recognition mechanism involving photo-induced electron transfer was interpreted theoretically by means of DFT and TDDFT calculations skillfully when NBDlyso comes into play under the acidic condition. This probe showed good ability to sense pH change in living cell image.

• 出版日期2016-5-12
• 单位山东大学; 泰山学院