Aurora B Overexpression Causes Aneuploidy and p21(Cip1) Repression during Tumor Development

作者：Gonzalez Loyola Alejandra; Fernandez Miranda Gonzalo; Trakala Marianna; Partida David; Samejima Kumiko; Ogawa Hiromi; Canamero Marta; de Martino Alba; Martinez Ramirez Angel; de Carcer Guillermo; Perez de Castro Ignacio; Earnshaw William C; Malumbres Marcos^*

来源：Molecular and Cellular Biology, 2015, 35(20): 3566-3578.

DOI：10.1128/MCB.01286-14

Aurora kinase B, one of the three members of the mammalian Aurora kinase family, is the catalytic component of the chromosomal passenger complex, an essential regulator of chromosome segregation in mitosis. Aurora B is overexpressed in human tumors although whether this kinase may function as an oncogene in vivo is not established. Here, we report a new mouse model in which expression of the endogenous Aurkb locus can be induced in vitro and in vivo. Overexpression of Aurora B in cultured cells induces defective chromosome segregation and aneuploidy. Long-term overexpression of Aurora B in vivo results in aneuploidy and the development of multiple spontaneous tumors in adult mice, including a high incidence of lymphomas. Overexpression of Aurora B also results in a reduced DNA damage response and decreased levels of the p53 target p21(Cip1) in vitro and in vivo, in line with an inverse correlation between Aurora B and p21(Cip1) expression in human leukemias. Thus, overexpression of Aurora B may contribute to tumor formation not only by inducing chromosomal instability but also by suppressing the function of the cell cycle inhibitor p21(Cip1).

• 出版日期2015-10