科研之友
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摘要
It is necessary to develop rapid, simple and accurate detection method for Escherichia coli (E. coli) due to its widely distributed pathogenic bacteria. Herein, we prepared AgBr nanoparticles (NPs) anchored 3D nitrogen doped graphene hydrogel (3DNGH) nanocomposites with an exceptionally large accessible surface by a simple hydrothermal approach. The as-prepared 3DNGH porous nanocomposite not only showed better conductivity than that of 3D graphene due to introducing nitrogen element into graphene framework, but also provided a high loading volume for immobilizing luminol. Meanwhile the anchored AgBr NPs served as the catalyst can effectively enhance the ECL behavior of luminol. And the resulting luminol/AgBr/3DNGH exhibited more excellent ECL performances, which was about 2, 3, 8 times enhanced respectively, comparing to luminol/AgBr/ 3DGH, luminol/3DNGH and luminol/AgBr/2DNG. Further, the multifunctional nanoarchitecture was used as the all-solid-state ECL platform for fabricating Escherichia coli aptasensors via glutaraldehyde as crosslinking agent between amine-functionalized E. coli aptamer and luminol/AgBr/3DNGH. Based on the steric hindrance mechanism that E.coli can significantly decrease the ECL intensity, the proposed aptasensor displayed a linear response for E.coli in the range from 0.5 to 500 cfu/mL with an extremely low detection limit of 0.17 cfu/mL (S/N). In addition, this ECL aptasensor possessed great advantages including the simple operation process, lowcost and sensitivity, which provided a promising approach for the E.coli detection in biomedical, food detection and environmental analysis.
- 出版日期2017-11-15
- 单位江苏大学
