科研之友
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摘要
Jasmonate (JA) and ethylene are involved in the regulation of the aluminum (Al)-induced growth inhibition. Although it has been reported that JA enhances Al-induced root-growth inhibition, its role in the regulation of growth interplaying with ethylene is still not well understood. In this study, we investigated the mechanism underlying the effect of apple MdMYC2 transcription factor on Al stress. Overexpression lines were used for functional analysis. Real-time quantitative RT-PCR was used to examine the expression level of ethylene responsive genes. ChIP-PCR, EMSA, and Y1H assays were used to test whether MdMYC2-GST fusion protein could directly bind to MdERF3 promoter. Transient transactivation assays in tobacco leave were conducted to confirm whether MdMYC2 positively regulated the expression of MdERF3. MdMYC2 negatively regulated Al tolerance with up-regulating the expression of ethylene responsive genes. Moreover, MdMYC2 was observed to bind to the promoter of MdERF3, a positive regulator of ethylene biosynthesis, and directly activated its transcription. And applying the antagonist of ethylene biosynthesis, AVG, alleviated MdMYC2-modulated growth inhibition in Al stress. We consider that MdMYC2 protein directly interacts and promotes the transcript of MdERF3 to affect ethylene biosynthesis, thereby regulating the Al-mediated stress response. Our findings provide a deeper understanding of the crosstalk between JA and ethylene as well as JA-mediated growth inhibition in apple.
- 出版日期2017-9
- 单位山东农业大学
