Acteoside reduces testosterone by inhibiting cAMP, p450scc, and StAR in rat Leydig cells

作者：Liu, Shuqiang; Zhang, Junwen; Li, Weixuan; Zhang, Tianxiang; Hu, Defu^*

来源：Molecular & Cellular Toxicology, 2015, 11(1): 11-17.

DOI：10.1007/s13273-015-0002-x

• Acteoside
• cAMP
• p450scc
• StAR
• Testosterone
• Leydig cells

The present study investigated the in vivo and in vitro effects of acteoside on testosterone production in Sprague-Dawley (SD) rats. The in vivo experiment revealed that acteoside reduced the testosterone level in serum significantly (P<0.05). The in vitro experiment also illustrated that acteoside significantly reduced testosterone production in SD rat Leydig cells in primary culture (P<0.05). Enzyme-linked immunosorbent assay results demonstrated that acteoside significantly reduced the cyclic adenosine 3',5'-monophosphate (cAMP) level (P<0.05), and Western blot analysis showed that acteoside significantly reduced cholesterol side-chain cleavage enzyme (p450scc) and steroidogenic acute regulatory (StAR) expression (P<0.05). Hoechst 33342 staining and Western blotting showed that acteoside did not induce apoptosis in Leydig cells. Together, these results suggest that the acteoside-induced reduction in testosterone production in rat may be at least partially due to down-regulation of cAMP, p450scc, and StAR, but not apoptosis.

• 出版日期2015-3-31
• 单位北京林业大学