An amperometric biosensor based on laccase immobilized onto MnO(2)NPs/cMWCNT/PANI modified Au electrode

作者：Rawal Rachna; Chawla Sheetal; Malik Poonam; Pundir C S^*

来源：International Journal of Biological Macromolecules, 2012, 51(1-2): 175-181.

DOI：10.1016/j.ijbiomac.2011.11.022

• Phenolic compounds
• Laccase
• Amperometric biosensor
• Ganoderma sp.
• MnO2 NPs
• cMWCNT

A method is described for construction of an amperometric biosensor for detection of phenolic compounds based on covalent immobilization of laccase (Lac) onto manganese dioxide nanoparticles (MnO(2)NPs) decorated carboxylated multiwalled carbon nanotubes (cMWCNTs)/PANI composite electrodeposited onto a gold (Au) electrode through N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) chemistry. The modified electrode was characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The biosensor showed optimum response at pH 5.5 (0.1 M sodium acetate buffer) and 35 degrees C, when operated at 0.3 V vs. Ag/AgCl. Linear range, response time, detection limit were 0.1-10 mu M (lower concentration range) and 10-500 mu M (higher concentration range), 4s and 0.04 mu M, respectively. Biosensor measured total phenolic content in tea leaves extract. The enzyme electrode was used 150 times over a period of 5 months.

• 出版日期2012-8