Anti-ribosomal P antibodies are associated with elevated circulating IFN alpha and IL-10 levels in systemic lupus erythematosus patients

作者：Mozo L^*; Lopez P; Caminal Montero L; Rodriguez Carrio J; Suarez A

来源：Lupus, 2014, 23(14): 1477-1485.

DOI：10.1177/0961203314546020

• Anti-ribosomal protein (RibP) antibodies
• SLE
• IL-10
• IFN alpha
• anti-dsDNA
• disease activity

Objective: The objective of this paper is to analyze the relationship of anti-protein ribosomal P (RibP) antibodies with circulating levels of IFN alpha, TNF alpha, IFN gamma, IL-17 and IL-10 in SLE. Disease activity and other systemic lupus erythematosus (SLE) features were also analyzed. Methods: Anti-RibP and other SLE-related antinuclear antibodies (ANA) were determined by fluoro-enzyme immunoassay in the sera of 107 SLE patients. Circulating cytokines were quantified by flow cytometry (IFN alpha, IL-10 and IL-17) or ELISA (TNF alpha and IFN gamma). Results: Anti-RibP-positive patients (14.9%) displayed significantly higher serum levels of IFN alpha (p = 0.023) and IL-10 (p = 0.016) than their negative counterparts. This cytokine upregulation was independent of the presence of other ANA even though, in our patient cohort, anti-dsDNA was found to be associated with anti-RibP (OR, CI 95%: 6.03, 1.32-27.93, p = 0.021) and to correlate with IL-10 levels (r = 0.204, p = 0.036). In fact, patients positive for anti-RibP but negative for anti-dsDNA exhibited the highest amounts of both IL-10 and IFN-alpha that were not related to disease activity since these patients showed lower SLEDAI than patients also positive for anti-dsDNA (p = 0.018). Anti-RibP positivity was also associated with early diagnosis, hypocomplementemia and leukopenia. Conclusions: Presence of anti-RibP was found to be related to increased serum IFN alpha and IL-10 levels independently of both antibody status and disease activity.

• 出版日期2014-12