Monitoring of duplex and triplex formation by F-19 NMR using oligodeoxynucleotides possessing 5-fluorodeoxyuridine unit as F-19 signal transmitter

作者：Tanabe Kazuhito^*; Sugiura Masaaki; Nishimoto Sei ichi

来源：Bioorganic & Medicinal Chemistry, 2010, 18(18): 6690-6694.

DOI：10.1016/j.bmc.2010.07.066

• DNA
• F-19 NMR
• Higher order structures
• 5-Fluorodeoxyuridine

We prepared oligodeoxynucleotides (ODNs) possessing a 5-fluorodeoxyuridine (5-FU) unit as a F-19-signal transmitter, and characterized their structures including single strand, duplex, and triplex using F-19 NMR. The change in chemical shift induced by incorporation of 5-FU into the ODNs and the formation of higher order structures allowed monitoring of structural changes. Data from UV melting experiments and CD spectra were consistent with the spectral changes in the NMR studies. These F-19-labeled ODNs may be promising molecular probes for the identification of DNA structures in complicated biological conditions.

• 出版日期2010-9-15