Depth-resolved cellular microrheology using HiLo microscopy

作者：Michaelson Jarett^*; Choi Heejin; So Peter; Huang Hayden

来源：Biomedical Optics Express, 2012, 3(6): 1241-1255.

It is increasingly important to measure cell mechanical properties in three-dimensional environments. Particle tracking microrheology (PTM) can measure cellular viscoelastic properties; however, out-of-plane data can introduce artifacts into these measurements. We developed a technique that employs HiLo microscopy to reduce out-of-plane contributions. This method eliminated signals from 90% of probes 0.5 mu m or further from the focal plane, while retaining all in-plane probes. We used this technique to characterize live-cell bilayers and found that there were significant, frequency-dependent changes to the extracted cell moduli when compared to conventional analysis. Our results indicate that removal of out-of-plane information is vital for accurate assessments of cell mechanical properties.

• 出版日期2012-6-1