A New Unusual Delta(11(12))-Oleane Triterpene and Anti-complementary Triterpenes from Prunella vulgaris Spikes

作者：Du Dongsheng; Cheng Zhihong^*; Chen Daofeng

来源：Natural Product Communications, 2012, 7(4): 501-505.

• Prunella vulgaris
• Anti-complementary activity
• Triterpenes
• 3 beta,13 beta-Dihydroxyolic-11-ene-28-oic acid

Anti-complementary activity-guided fractionation of the ethanolic extract of Prunella vulgaris spikes led to the isolation of a new, unusual Delta(11(12)) triterpene, 3 beta,13 beta-dihydroxyolic-11-ene-28-oic acid (1), along with thirteen known triterpenes (2-14). The structure of the new compound was established by 1D and 2D NMR spectroscopic analysis. All the isolates were evaluated for their anti-complementary activity against the classical pathway (CP) and alternative pathway (AP). Eight triterpenes (1-8) showed anti-complementary activity against CP and AP, with CH50 and AP(50) values of 0.15-0.37 mg/mL, and 0.29-0.53 mg/mL, respectively. Mechanism study using complement-depleted sera showed that oleanolic acid (2) acted selectively on Clq, C5, and C9 components, while 2 alpha-hydroxy oleanolic acid (3) interacted with Clq, C3, C5 and C9, and 2 alpha,3 alpha-dihydroxyolic-12-ene-28-oic acid (4) blocked Clq, C2, C3, C5 and C9.

• 出版日期2012-4
• 单位复旦大学