Mitochondrial (COI) and nuclear (EF-1 alpha) DNA variability of Rhopalosiphum padi and Rhopalosiphum nymphaeae (Hemiptera: Aphididae) in Lithuania

作者：Rakauskas Rimantas^*; Havelka Jekaterina; Bernotiene Rasa

来源：Biologia, 2014, 69(12): 1730-1741.

DOI：10.2478/s11756-014-0475-4

• Rhopalosiphum
• Mitochondrial DNA
• COI
• Nuclear DNA
• EF-1 alpha
• Europe

Partial sequences of mitochondrial COI and nuclear EF-1 alpha genes have been analysed across the Lithuanian samples of Rhopalosiphum padi and R. nymphaeae (Hemiptera: Aphididae). In addition, samples from the adjacent countries of the Eastern Baltic region of Europe (Latvia, Estonia and Poland) and available sequences from GenBank and Bold Systems were used. The range of intraspecific pairwise R. padi COI sample (n = 44) divergences (K2P model) was 0.00-2.49% (average 0.61%). Out of 10 haplotypes, one was predominant (n = 25), with Worldwide distribution. The range of intraspecific pairwise R. nymphaeae COI sample (n = 20) divergences (K2P model) was 0.00-1.31% (average 0.22%). Out of 6 haplotypes, one was predominant (n = 13), sampled in Lithuania, Ukraine and Turkey (Asian part). The range of intraspecific pairwise sample divergences (K2P model) of EF-1 alpha fragment was 0.00-0.40% (average 0.10%) for R. padi (n = 20) and 0.00-0.60% (average 0.13%) for R. nymphaeae (n = 17). Two COI haplotypes (including the Worldwide distributed one) and three EF-1 alpha haplotypes (including the most common one) were detected in Lithuanian populations of R. padi. The present study suggests that R. padi populations from the Eastern Baltic region are predominantly holocyclic.

• 出版日期2014-12