Binding affinity of ssDNA is improved by attachment of dsDNA regions

作者：Ida Hiroyuki; Tachibana Akira^*; Tanabe Toshizumi

来源：Journal of Bioscience and Bioengineering, 2014, 118(3): 239-241.

DOI：10.1016/j.jbiosc.2014.02.023

• MicroRNA inhibitor
• LidNA
• ssDNA binding affinity
• Surface plasmon resonance
• Fluorescence polarization

LidNA, a microRNA inhibitor consisting of a microRNA binding ssDNA region sandwiched between dsDNA regions had higher affinity to target oligonucleotides than that without dsDNA region. This enhancement in affinity was found to be owing to the suppressed mobility of ssDNA region by the presence of dsDNA regions.

• 出版日期2014-9