Impedimetric Detection of Hairpin Ribozyme Activity

作者：Piekielska Katarzyna; Gebala Magdalena; Gwiazda Slawomir; Mueller Sabine; Schuhmann Wolfgang^*

来源：Electroanalysis, 2011, 23(1): 37-42.

DOI：10.1002/elan.201000640

• Ribozyme
• RNA
• Electrochemical impedance spectroscopy
• Label free detection

Electrochemical impedance spectroscopy is successfully utilized for label-free monitoring of the cleavage reaction of a RNA substrate by a complementary hairpin ribozyme. The formation of the RNA substrate/ribozyme complex is increasing the negative charge at the interface and is modulating the kinetic of the redox conversion of a negatively charged redox mediator (e.g. [Fe(CN)(6)](3-/4-)) hence leading to an increase in the charge transfer resistance. Upon addition of bivalent cations such as Mg(2+) ions the conformation of the ribozyme is changed and the catalytic cleavage of the RNA is monitored by a decrease of the charge transfer resistance.

• 出版日期2011-1