Antihypertensive effect and purification of an ACE inhibitory peptide from sea cucumber gelatin hydrolysate

作者：Zhao Yuanhui; Li Bafang; Liu Zunying; Dong Shiyuan; Zhao Xue; Zeng Mingyong^*

来源：Process Biochemistry, 2007, 42(12): 1586-1591.

DOI：10.1016/j.procbio.2007.08.011

• sea cucumber
• gelatin
• hydrolysate
• ACE inhibitory peptide
• renal hypertensive rat
• antihypertensive effect

Gelatin from the sea cucumber (Acaudina molpadioidea) was hydrolyzed sequentially with bromelain and alcalase. The hydrolysate was fractionated into three ranges of molecular weight (GH-I, <10 kDa; GH-II, <5 kDa; GH-III, <1 kDa) using an ultrafiltration membrane bioreactor system. The GH-III brought about a high angiotensin-l-converting enzyme (ACE) inhibitory activity, with IC50 value of 0.35 mg/ml. An ACE inhibitory peptide was isolated from the GH-III, using the chromatographic methods including ion-exchange chromatography, gel filtration and reversed phase high-performance liquid chromatography. The purified ACE inhibitory peptide with a molecular weight of 840 Da consisted of five main amino acids (Glu, Asp, Pro, Gly and Ala), and its IC50 value was 0.0142 mg/ml. This sea cucumber gelatin hydrolysate (GH-III) was used as drinks administered to renal hypertensive rats (RHR) for 1 month. The systolic blood pressure and diastolic blood pressure of the RHR were significantly reduced, which indicates an antihypertensive effect by oral administration.

• 出版日期2007-12
• 单位中国海洋大学