ade9 is an allele of SER1 and plays an indirect role in purine biosynthesis

作者：Buc PS; Rolfes RJ^*

来源：Yeast, 1999, 15(13): 1347-1355.

DOI：10.1002/(SICI)1097-0061(19990930)15:13<1347::AID-YEA461>3.3.CO;2-1

• purine biosynthesis
• serine biosynthesis
• one-carbon metabolism
• ADE9
• SER1

In this study we demonstrate that ade9 plays an indirect role in purine biosynthesis as a non-functional allele of SER1 in Saccharomyces cerevisiae. The SER1 locus, encoding 3-phosphoserine aminotransferase required for serine biosynthesis, is located on chromosome XV and resides approximately where ade9 had previously been mapped genetically. A minimal functional construct of SER1 is necessary and sufficient to complement both the adenine- and serine-requiring phenotypes of ade9 strains. In addition, adequate exogenous serine levels mask the adenine phenotype of ade9. A disruption of SER1 behaves in the same manner phenotypically as the original ade9 strain. Therefore, ade9 can be more accurately described as an allele of SER1.