ANTICANCER ACTIVITIES OF EXTRACTS OF Peristrophe bicalyculata (RETZ) NEES

作者：Abdulazeez Mansurah A^*; Ibrahim Sani; Ameh D A; Ayo J O; Carvalho L J C

来源：Romanian Biotechnological Letters, 2013, 18(1): 7995-8006.

• Peristrophe bicalyculata
• Sulphorhodamine B assay
• anticancer
• apoptosis
• necrosis
• andrographolide

The anticancer activity of leaf extracts of Peristrophe bicalyculata was evaluated. Results showed that the methanolic ethylacetate fraction with an IC50 of 15.60 +/- 0.52 mu g/ml was potentially very toxic against human mouth epidermal carcinoma (KB) cells. After partial purification of the methanolic ethylacetate fraction, seven fractions were obtained; five of which were inactive (IC50 values above 500 mu g/ml) and one moderately toxic (IC50 = 235 +/- 33.05 mu g/ml), hence not considered. The IC50 value of the most cytotoxic fraction (fraction 5) (3.50 +/- 0.21 mu g/ml) was not different from that of cisplastin (3.32 +/- 0.09 mu g/ml), but was significantly (P<0.05) higher than the value obtained for doxorubicin (0.43 +/- 0.02 mu g /ml) and vincristine (0.05 +/- 0.01 mu g/ml). The partially-purified fraction induced apoptosis in KB cells after 24 and 48 hours in a dose-dependent manner. The percentage of apoptotic cells in cultures treated with this fraction was significantly (P<0.05) higher than that in control cultures, but lower than cultures treated with cisplastin. The GC-MS analysis of the fraction indicated an abundance of andrographolide 2(3H)-furanone and aromadendrene oxide, both of which possess anticancer properties. The results provide scientific evidence supporting the anticancer activity of the methanolic ethylacetate fraction of the plant. In conclusion, the methanolic ethylacetate fraction of Peristrophe bicalyculata is a potential source of chemotherapeutic agent.

• 出版日期2013-2