Development of a SCAR (Sequence-characterised amplified region) marker for acid resistance-related gene in Lactobacillus plantarum

作者：Liu, Shu-Wen; Li, Kai; Yang, Shi-Ling; Tian, Shu-Fen; He, Ling^*

来源：Extremophiles, 2015, 19(2): 355-361.

DOI：10.1007/s00792-014-0721-2

• Lactobacillus plantarum
• Acid-resistant gene
• RAPD marker
• SCAR marker

A sequence characterised amplified region marker was developed to determine an acid resistance-related gene in Lactobacillus plantarum. A random amplified polymorphic DNA marker named S116-680 was reported to be closely related to the acid resistance of the strains. The DNA band corresponding to this marker was cloned and sequenced with the induction of specific designed PCR primers. The results of PCR test helped to amplify a clear specific band of 680 bp in the tested acid-resistant strains. S116-680 marker would be useful to explore the acid-resistant mechanism of L. plantarum and to screen desirable malolactic fermentation strains.

• 出版日期2015-3
• 单位西北农林科技大学; 天津市农业科学院