Dispersive solid phase extraction/fluorescence analysis of riboflavin using sepiolite as sorbent

作者：Mateos Rocio; Vera Lopez Soledad; Maria Diez Pascual Ana; Paz San Andres Maria

来源：Applied Clay Science, 2018, 163: 279-290.

DOI：10.1016/j.clay.2018.07.033

• Sepiolite
• Riboflavin
• Dispersive solid phase extraction
• Surfactants
• Food samples

The use of a natural clay, sepiolite (Sep), and graphene (G)/Sep mixtures as sorbents for the solid phase extraction in dispersive mode (dSPE) with fluorescence detection of a biologically interesting molecule, riboflavin, is reported. The retention of riboflavin by Sep is quantitative with different volumes of the sample and 10-50 mg of the clay. The desorption process has been performed using aqueous solutions of surfactants of different nature: anionic sodium dodecylsulphate (SDS), cationic hexadecyltrimethylammonium chloride (CTAC) and non-ionic polyoxyethylene-23-lauryl eter (Brij L23). The non-ionic surfactant provides the highest riboflavin recoveries; upon optimization of the process, that takes less than half an hour, an extraction yield of 93% was attained using 40 mM Brij L23. The developed method has been applied to the analysis of beer, soya drink and infant milk samples with excellent accuracy (recoveries of 100%) and very good precision, avoiding the interferences of the matrix components that hinder the direct fluorescence analysis of riboflavin.

• 出版日期2018-10