Automated Synthesis of Arabinoxylan-Oligosaccharides Enables Characterization of Antibodies that Recognize Plant Cell Wall Glycans

作者：Schmidt Deborah; Schuhmacher Frank; Geissner Andreas; Seeberger Peter H; Pfrengle Fabian^*

来源：Chemistry - A European Journal, 2015, 21(15): 5709-5713.

DOI：10.1002/chem.201500065

• arabinoxylan
• carbohydrates
• microarray
• plant cell wall
• solid-phase synthesis

Monoclonal antibodies that recognize plant cell wall glycans are used for high-resolution imaging, providing important information about the structure and function of cell wall polysaccharides. To characterize the binding epitopes of these powerful molecular probes a library of eleven plant arabinoxylan oligosaccharides was produced by automated solid-phase synthesis. Modular assembly of oligoarabinoxylans from few building blocks was enabled by adding (2-naphthyl) methyl (Nap) to the toolbox of orthogonal protecting groups for solid-phase synthesis. Conjugation-ready oligosaccharides were obtained and the binding specificities of xylan-directed antibodies were determined on microarrays.

• 出版日期2015-4-7