Hydroxyethyl acrylate-based copolymer-immobilized liposomes as UV and thermo dual-triggerable carriers

作者：Kim Jin Chul^*

来源：European Journal of Lipid Science and Technology, 2015, 117(1): 45-54.

DOI：10.1002/ejlt.201400254

• Dual-triggerable carrier
• Liposomes
• Poly(hydroxyethyl acrylate-co-coumaryl acrylate-co-2-ethylhexyl acrylate)

UV- and temperature-triggerable liposomes were prepared by modifying the surface of egg phosphatidylcholine (EPC) liposomes with poly(hydroxyethyl acrylate-co-coumaryl acrylate-co-2-ethylhexyl acrylate) (P(HEA-CA-EHA)). P(HEA-CA-EHA)s were prepared by a free radical polymerization using reaction mixtures where HEA/CA/EHA molar ratio was 97.5:0.5:2 and 97:1:2. The CA residue of P(HEA-CA-EHA) (97.5:0.5:2) and that of P(HEA-CA-EHA) (97:1:2) were readily dimerized under the irradiation of UV light (254nm, 6W). P(HEA-CA-EHA) (97:1:2) exhibited a lower critical solution temperature (LCST) around 37 degrees C but P(HEA-CA-EHA) (97.5:0.5:2) showed no LCST. EPC liposomes incorporating the copolymers of which EPC/copolymer ratio was 200:1-10:1 were prepared by bath-type sonication. The fluorescence quenching degree was 65-92%, depending on the EPC/copolymer ratio. The liposomes released their content in response to the irradiation of the UV light, and the photo-sensitivity of liposomes incorporating P(HEA-CA-EHA) (97:1:2) was somewhat higher than that of liposomes incorporating P(HEA-CA-EHA) (97.5:0.5:2). Liposomes incorporating P(HEA-CA-EHA) (97:1:2) exhibited a temperature-responsive release, but liposomes bearing P(HEA-CA-EHA) (97.5:0.5:2) did not. The UV- and temperature-triggered release property of the liposomes was possibly due to the photo- and thermo-sensitive property of the copolymer immobilized on the liposomal surface.
Practical applications: The liposomes incorporating P(HEA-CA-EHA) could be applied in controlled release drug delivery, releasing the cargo in response to ultra-violet light irradiation and to temperature change.

• 出版日期2015-1