A golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis

作者：Wang Helen X; Weerasinghe Ravisha R; Perdue Tony D; Cakmakci Nihal G; Taylor J Philip; Marzluff William F; Jones Alan M^*

来源：Molecular Biology of the Cell, 2006, 17(10): 4257-4269.

DOI：10.1091/mbc.E06-01-0046

Signal transduction involving heterotrimeric G proteins is universal among fungi, animals, and plants. In plants and fungi, the best understood function for the G protein complex is its modulation of cell proliferation and one of several important signals that are known to modulate the rate at which these cells proliferate is D-glucose. Arabidopsis thaliana seedlings lacking the beta subunit (AGB1) of the G protein complex have altered cell division in the hypocotyl and are D-glucose hypersensitive. With the aim to discover new elements in G protein signaling, we screened for gain-of-function suppressors of altered cell proliferation during early development in the agb1-2 mutant background. One agb1-2dependent suppressor, designated sgb1-1(D) for suppressor of G protein betal (agb1-2), restored to wild type the altered cell division in the hypocotyl and sugar hypersensitivity of the agb1-2 mutant. Consistent with AGB1 localization, SGB1 is found at the highest steady-state level in tissues with active cell division, and this level increases in hypocotyls when grown on D-glucose and sucrose. SGB1 is shown here to be a Golgi-localized hexose transporter and acts genetically with AGB1 in early seedling development.

• 出版日期2006-10