Aluminium foil as an alternative substrate for the spectroscopic interrogation of endometrial cancer

作者：Paraskevaidi Maria; Morais Camilo L M; Raglan Olivia; Lima Kassio M G; Paraskevaidis Evangelos; Martin Hirsch Pierre L; Kyrgiou Maria; Martin Francis L^*

来源：Journal of Biophotonics, 2018, 11(7): UNSP e201700372.

DOI：10.1002/jbio.201700372

• attenuated total reflection
• infared
• ATR-FTIR spectroscopy
• substrate
• aluminium foil
• low-E slide
• plasma
• serum
• endometrial cancer
• gynaecological cancer

Biospectroscopy has the potential to investigate and characterize biological samples and could, therefore, be utilized to diagnose various diseases in a clinical environment. An important consideration in spectrochemical studies is the cost-effectiveness of the substrate used to support the sample, as high expense would limit their translation into clinic. In this paper, the performance of low-cost aluminium (Al) foil substrates was compared with the commonly used low-emissivity (low-E) slides. Attenuated total reflection-Fourier transform infrared spectroscopy was used to analyse blood plasma and serum samples from women with endometrial cancer and healthy controls. The 2 populations were differentiated using principal component analysis with support vector machines with 100% sensitivity in plasma samples (endometrial cancer = 70; healthy controls = 15) using both Al foil and low-E slides as substrates. The same sensitivity results (100%) were achieved for serum samples (endometrial cancer=60; healthy controls = 15). Specificity was found higher using Al foil (90%) in comparison to low-E slides (85%) and lower using Al foil (70%) in comparison to low-E slides in serum samples. The establishment of Al foil as low-cost and highly performing substrate would pave the way for large-scale, multicentre studies and potentially for routine clinical use.

• 出版日期2018-7