mTORC1 regulates mannose-6-phosphate receptor transport and T-cell vulnerability to regulatory T cells by controlling kinesin KIF13A

作者：Ahmed Khawaja Ashfaque; Xiang Jim^*

来源：Cell Discovery, 2017, 3(1): UNSP 17011.

DOI：10.1038/celldisc.2017.11

• M6PR
• T-cell fate
• mTORC1
• KIF13A
• T-reg cell
• apoptosis

Mannose-6-phosphate receptor (M6PR) that facilitates cellular uptake of M6P-bearing proteins, including serine-protease granzyme-B (Gzm-B) has an important role in T-cell activation, migration and contraction. However, molecular mechanisms controlling M6PR expression in T cells remain poorly understood. Here, we show that M6PR expression on T cells is distinctively controlled by two common.-chain cytokines interleukin-2 (IL-2) and IL-7, and the differential M6PR expression is not caused by an altered synthesis of M6PR protein, but is a result of distinct regulation of kinesin-3 motor-protein KIF13A that transport M6PR onto cell surfaces. Using signaling pathway-specific inhibitors, we determine that IL-2 and IL-7 distinctly regulate KIF13A and beta 1-adaptin and cell-surface M6PR by controlling a kinase mammalian target of rapamycin complex-1 (mTORC1). Inflammatory cytokine IL-2 and prosurvival cytokine IL-7 induce strong and weak activation of mTORC1, leading to up-and downregulation of motor-protein KIF13A and KIF13A-motorized M6PR on T cells, and formation of IL-2 and IL-7 effectors with M6PR(high) and M6PR(low) cell-surface expression, respectively. Inhibition of mTORC1 by rapamycin reduces T-cell expression of KIF13A and cell-surface M6PR, and increases T-cell survival in Listeria monocytogenes-infected mice. Using regulatory T (T-reg)-cell-enriched mouse tumor model, we determine that M6PR(high) IL-2 effectors but not M6PR(low) IL-7 effectors adoptively transferred into tumors are vulnerable to T-reg Gzm-B-mediated cell apoptosis. Inhibition of mTORC1 or small interfering RNA-mediated knockdown of KIF13A or M6PR renders IL-2 effectors refractory to T-reg Gzm-B lethal hit. Overall, our data offer novel mechanistic insights into T-cell M6PR regulation, and T-reg-resistant/T-reg-susceptible phenomenon. Furthermore, regulation of T-cell fate vis-a-vis T-reg suppression via the mTORC1-KIF13A-M6PR axis provides a proof of concept for therapeutic strategies to target cancer, infectious and autoimmune diseases.

• 出版日期2017-4-25
• 单位Saskatchewan; Saskatoon