Molecular characterization of DNA beta molecules associated with Tobacco leaf curl Yunnan virus

作者：Cui XF; Xie Y; Zhou XP^*

来源：Journal of Phytopathology, 2004, 152(11-12): 647-650.

DOI：10.1111/j.1439-0434.2004.00911.x

• Tobacco leaf curl Yunnan virus
• DNA beta
• variability

Tobacco leaf curl Yunnan virus (TbLCYNV) isolates Y136 and Y143 were both shown to contain DNAbeta molecules. Seven DNAbeta molecules, each consisting of 1334-1341 nucleotides, were cloned and characterized. Sequence comparison showed that they were of two main types: type 1 included five DNAbeta molecules with 75-99% overall nucleotide sequence identity, and type 2 included two DNAbeta molecules with >99% overall nucleotide sequence identity. Only 52-54% nucleotide identity was found between the two types of DNAbeta molecules. The two isolates were associated with both types of DNAbeta. A relationship dendrogram analysis showed that type 1 DNAbeta molecules clustered with the DNAbeta molecules of Tomato yellow leaf curl China virus, whereas type 2 DNAbeta molecules clustered with the DNAbeta of Tomato yellow leaf curl Thailand virus. Evidence was also provided that type 2 DNAbeta molecules may have arisen by recombination between the DNAbeta of Tobacco curly shoot virus and another unidentified virus. To date, only a single type of DNAbeta molecule had been found to be associated with one begomovirus. The possible reasons of TbLCYNV associated with two types of DNAbeta molecules are discussed.

• 出版日期2004-12
• 单位浙江大学