A Rapid New Approach for the Quality Evaluation of the Folk Medicine Dianbaizhu Based on Chemometrics

作者：Liu Zizhen; Jiang Rui; Xie Meng; Xu Guanling; Liu Weirui; Wang Xiaohong; Lin Hongying; Lu Jianqiu^*; She Gaimei

来源：Chemical & Pharmaceutical Bulletin, 2014, 62(11): 1083-1091.

DOI：10.1248/cpb.c14-00407

• Dianbaizhu
• Chinese folk medicine
• quality evaluation
• chemometrics
• HPLC coupled with diode array detector

Dianbaizhu, a folk medicine from Gaultheria leucocarpa BLUME var. yunnanensis (FRANCH.) T. Z. Hsu & R. C. FANG (Ericaceae) used as an antirheumatic, has multiple plant origins and officinal parts. A rapid high-performance liquid chromatography with diode array detector (HPLC-DAD) method was established for the simultaneous determination of the characteristic ingredient methyl benzoate-2-O-beta-D-glucopyranosyl(1 -> 2) [O-beta-D-xylopyranosyl(1 -> 6)]-O-beta-D-glucopyranoside and seven bioactive constituents in eight Gaultheria species. This chromatographic method is precise, accurate, and stable. Kruskal Wallis analysis, hierarchical cluster analysis, and factor analysis were used to analyze the content of reference compounds in different Gaultheria species and officinal parts. The analyses showed significant differences (p<0.05) in Gaultheria species but few differences (p>0.05) in their medicinal parts. G. leucocarpa var. yunnanensis appeared to the best among the Gaultheria species tested for the treatment of rheumatic diseases. Taken together, the results show that this simultaneous quantification of multiple active constituents using HPLC-DAD combined with chemometrics can be reliably applied to evaluate the quality of Dianbaizhu.

• 出版日期2014-11
• 单位北京中医药大学