Antimicrobial Activity of Diterpenes from Viguiera arenaria against Endodontic Bacteria

作者：Carvalho Tatiane C; Simao Marilia R; Ambrosio Sergio R; Furtado Niege A J C; Veneziani Rodrigo C S; Heleno Vladimir C G; Da Costa Fernando B; Gomes Brenda P F A; Souza Maria Gorete M; dos Reis Erika Borges; Martins Carlos H G^*

来源：Molecules, 2011, 16(1): 543-551.

DOI：10.3390/molecules160100543

• antimicrobial activity
• endodontic bacteria
• pimarane diterpenes
• structural requirements
• Viguiera arenaria

Six pimarane-type diterpenes isolated from Viguiera arenaria Baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. The microdilution method was used for the determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Porphyromonas gingivalis, Prevotella nigrescens, Prevotella intermedia, Prevotella buccae, Fusobacterium nucleatum, Bacteroides fragilis, Actinomyces naeslundii, Actinomyces viscosus, Peptostreptococcus micros, Enterococcus faecalis and Aggregatibacter actinomycetemcomitans. The compounds ent-pimara-8(14), 15-dien-19-oic acid, its sodium salt and ent-8(14), 15-pimaradien-3 beta-ol were the most active, displaying MIC values ranging from 1 to 10 mu g mL(-1). The results also allow us to conclude that minor structural differences among these diterpenes significantly influence their antimicrobial activity, bringing new perspectives to the discovery of new chemicals for use as a complement to instrumental endodontic procedures.

• 出版日期2011-1