Alleviation of silver toxicity by calcium chloride (CaCl2) in Lemna gibba L.

作者：Oukarroum Abdallah^*; Gaudreault Marie Helene; Pirastru Laura; Popovic Radovan

来源：Plant Physiology and Biochemistry, 2013, 71: 235-239.

DOI：10.1016/j.plaphy.2013.07.019

• Lemna gibba
• Silver ions
• Calcium
• Frond abscission
• Reactive oxygen species

The toxicity effects of silver (Ag) and the protective role of calcium chloride (CaCl2) was studied in Lemna gibba L. (L gibba) plants. Silver speciation showed that silver toxicity in L gibba culture medium can be attributed to free ionic Ag+ concentration. Frond abscission, intracellular reactive oxygen species (ROS) formation and intracellular uptake of Ag+ were investigated when L gibba plants were exposed to AgNO3 concentrations (0.5, 1, 5, and 10 mu M) supplemented or not by 10 mu M CaCl2. An increase in frond abscission, intracellular ROS and intracellular uptake of Ag+ were detected in L gibba plants for all tested concentrations of AgNO3 after 24 h treatment. However, addition of 10 mu M CaCl2 to the L gibba culture medium reduced the toxic effects of Ag by decreasing silver uptake into the plant and intracellular ROS formation. The results suggest that Ag-induced toxicity was attributed to Ag+ accumulation and chloride was able to protect L. gibba plants against Ag toxicity by formation of complexes with Ag and then alleviation of the metal induced oxidative stress.

• 出版日期2013-10