A Pycnoporus sanguineus laccase for denim bleaching and its comparison with an enzymatic commercial formulation

作者：Magdalena Iracheta Cardenas Maria; Rocha Pena Mario A; Galan Wong Luis J; Arevalo Nino Katiushka; Eduardo Tovar Herrera Omar

来源：Journal of Environmental Management, 2016, 177: 93-100.

DOI：10.1016/j.jenvman.2016.04.008

• CIE lab
• Laccase activity
• Denim bleaching
• Dye decolorization
• Pycnoporus

A laccase from the basidiomycete Pycnoporus sanguineus strain RVAN5 was evaluated for its ability to decolorize synthetic dyes and denim bleaching. Dye color reduction and denim bleaching were monitored at different dye concentrations and incubation times. Dye decolorization by Pycnoporus sanguineus fungal crude extract (FCE) ranged from 80 to 96% within 2-4 h at 25-65 degrees C. Comparable results were obtained when violuric acid (VA) was added as mediator to the FCE, however, the number of decolorized dyes increased significantly. Dye decolorization rates with VA varied of initial and final optical density (595 nm) values of 2.5-3.0 and 0.2-0.02, respectively. P. sanguineus FCE had no substantial effect on denim bleaching when used alone, notwithstanding, the mixture of FCE with VA (10 mM) showed significant denim color reduction values and considerably higher than those obtained with a bleaching enzyme from a commercial formulation; CIElab values obtained with FCE/VA mixture were of Delta L=6.4, versus a Delta L 1.4 value obtained with an enzyme from commercial formulation.

• 出版日期2016-7-15