Anthraquinone Metabolites from Endophytic Nigrospora sp BM-2 and Optimization of Fermentation Medium for High Bostrycin Production

作者：Zhang, Hongqi; Deng, Zhangshuang^*; Guob, Zhiyong; Chenb, Jianfeng; Wangb, Junzhi; Zou, Kun

来源：Journal of Pure and Applied Microbiology, 2013, 7(4): 2489-2494.

• Endophytic Nigrospora sp.
• Anthraquinone
• Bostrycin
• Optimization
• Medium

Bioassay-guided separation led to identification of bostrycin(1), which was a potentially promising anti-tumor drug candidate, together with other four anthraquinone(2-5) derivatives from liquid culture of endophytic, Nigrospora sp. BM-2 isolated from Saccharum arundinaceum Retz. In order to obtain high bostrycin production, three single-factor experiments and a L-9(3(4)) orthogonal matrix experimental design have been employed in the cultivation of Nigrospora sp. BM-2. The optimal fermentation medium were glucose 20.0 g/L, yeast extract 8.0 g/L, MgSO4 0.05 g/L, KH2PO4 0.01g/L, NaCl 0.5 g/L. After 9 days of incubation under shake flask conditions at 120 rpm and 28 degrees C in optimal media, the highest amount of bostrycin was 86.25 +/- 7.23 mg/L in culture broth. Furthermore, enzymatic inhibitor as regulate factor in the biosynthesis of bostrycin was attempted to give adverse effects.

• 出版日期2013-12
• 单位武汉大学; 三峡大学