Differentiating sepsis from non-infectious systemic inflammation based on microvesicle-bacteria aggregation

作者：Herrmann I K^*; Bertazzo S; O'Callaghan D J P; Schlegel A A; Kallepitis C; Antcliffe D B; Gordon A C; Stevens M M

来源：Nanoscale, 2015, 7(32): 13511-13520.

DOI：10.1039/c5nr01851j

Sepsis is a severe medical condition and a leading cause of hospital mortality. Prompt diagnosis and early treatment has a significant, positive impact on patient outcome. However, sepsis is not always easy to diagnose, especially in critically ill patients. Here, we present a conceptionally new approach for the rapid diagnostic differentiation of sepsis from non-septic intensive care unit patients. Using advanced microscopy and spectroscopy techniques, we measure infection-specific changes in the activity of nano-sized cell-derived microvesicles to bind bacteria. We report on the use of a point-of-care-compatible microfluidic chip to measure microvesicle-bacteria aggregation and demonstrate rapid (<= 1.5 hour) and reliable diagnostic differentiation of bacterial infection from non-infectious inflammation in a double-blind pilot study. Our study demonstrates the potential of microvesicle activities for sepsis diagnosis and introduces microvesiclebacteria aggregation as a potentially useful parameter for making early clinical management decisions.

• 出版日期2015