Antileukemic activity of an arsenomolybdate in the human HL-60 and U937 leukemia cells

作者：Li, Chunyan; Cao, Hongqian; Sun, Jiaheng; Tian, Rui; Li, Dongbei; Qi, Yanfei^*; Yang, Wei^*; Li, Juan

来源：Journal of Inorganic Biochemistry, 2017, 168: 67-75.

DOI：10.1016/j.jinorgbio.2016.12.002

• Acute promyelocytic leukemia
• Polyoxometalates
• Arsenomolybdate
• Apoptosis

The antileukemic activity, mechanisms and serum albumin interactions of an arsenomolydate, K2Na[AsMo6O21(O2CCH2NH3)(3)]center dot 6H(2)O(1), was evaluated in the human leukemia HL-60 and U937 cells. The results indicated that 1 could inhibit the proliferation of both leukemia cell lines in a dose-dependent manner with the 50% lethal concentration (IC50) value of 8.61 mu M for HL-60 and 14.50 mu M for U937 at 24 h, compare to the positive controls, all-trans retinoic acid (ATRA) with IC50 value of 20.76 mu M and 14.85 mu M,and As2O3 with IC50 value of 6.40 mu M and 8.75 mu M at 24 h, respectively (P < 0.05). Furthermore, the anti-leukemia activity of compound 1 might be medicated by arresting the leukemic cells in the G(1) phase and inducing apoptosis via caspase-3 and bcl-2 regulatory proteins. Spectroscopic techniques results showed that the fluorescence of human serum albumin was quenched by compound 1, and the quenching mechanism was mainly static quenching. Compound 1 might be a potential medicinal candidate against acute promyelocytic leukemia.

• 出版日期2017-3
• 单位吉林大学