Detection of metallo-beta-lactamase-encoding genes among clinical isolates of Pseudomonas aeruginosa in northwest of Iran

作者：Yousefi Saber; Farajnia Safar^*; Nahaei Mohammd Reza; Akhi Mohammad Taghi; Ghotaslou Reza; Soroush Mohammad Hossein; Naghili Behrooz; Jazani Nima Hosseini

来源：Diagnostic Microbiology and Infectious Disease, 2010, 68(3): 322-325.

DOI：10.1016/j.diagmicrobio.2010.06.018

• Pseudomonas aeruginosa
• Metallo-beta-lactamases
• Polymerase chain reaction
• Double-disk synergy test
• Class 1 integron

The prevalence of metallo-beta-lactamase (MBL) production among 104 clinical isolates of Pseudomonas aeruginosa from northwest of Iran was investigated by phenotypic and polymerase chain reaction (PCR) methods. Thirty-nine (37.50%) of isolates were MBL positive by double-disk synergy test. Results of PCR revealed that 18(17.31%) and 6(5.77%) imipenem nonsusceptible isolates of P. aeruginosa carried bla(VIM) and bla(IMP) genes respectively, while 92.4% (62/67) of isolates contained class 1 integron gene. This is the first report of MBL-producing P. aeruginosa from northwest of Iran.

• 出版日期2010-11